Journal: Steroids

Article Title: Membrane-Initiated Nuclear Trafficking of the Glucocorticoid Receptor in Hypothalamic Neurons

doi: 10.1016/j.steroids.2017.12.005

Figure Lengend Snippet: Differential transcriptional effects of Dex and Dex-BSA. A. N42 cells transfected with GRE-TK-Luciferase for 24 hours were treated with Dex or Dex-BSA for 20 min. Hormone was removed and replaced with fresh media for 24 hours before lysis. **, p < 0.01; ns, p=0.064 compared to vehicle, two-tailed t-test; vehicles: DMSO and cyclodextrin in water (CD). B. Venn diagram for gene expression profiles in N42 cells following 24 hours of Dex and Dex-BSA treatment, from a Mouse OneArray® microarray (Phalanx Biotech Group) on 3 independent biological replicates. Genes reported show a ≥ 0.5-fold difference in expression (p < 0.05) compared to vehicle-treated cells.

Article Snippet: Mouse OneArray® microarray hybridization and data analysis were performed on the total RNA samples by Phalanx Biotech Group (Phalanx Biotech Group, San Diego, CA).

Techniques: Transfection, Luciferase, Lysis, Two Tailed Test, Gene Expression, Microarray, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Effects of Melatonin Administration on Intestinal Injury Caused by Abdominal Irradiation from Mice

doi: 10.3390/ijms22189715

Figure Lengend Snippet: The gene expression profile of the small intestine from mice following ABI. Mice were treated with melatonin (10 mg/kg) daily for 5 consecutive days by intraperitoneal injection before exposure to a dose of 14 Gy ABI. The small intestines were harvested at 3 days after radiation and DNA microarray analysis was performed. ( A ) The number of upregulated DEGs and downregulated DEGs for mice. The red-and-green chart shows the upregulated DEGs (red) and the downregulated DEGs (green). ( B ) Cluster analysis of similarity of genome-wide expression for mice. Every row represents a piece of chip; every line represents a gene probe. The red and green levels of probe signals indicate the level of upregulation (red) or downregulation (green). ( C ) Significantly enriched GO analysis (top10) of the DEGs for mice. The values of −log10 ( p -value) and −log10 (Q-value) show the relationship between gene expression and the relevant GO category. The y -axis represents the GO category and the x axis the −log10 ( p -value) and −log10 (Q-value). n = 3 mice/group.

Article Snippet: The aminoallyl-RNA probes were labelled with Cy5 hybridized for 16 h at 50 °C to a mouse oligo microarray (Mouse Whole Genome OneArray MOA2.1; Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques: Gene Expression, Injection, Microarray, Genome Wide, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Effects of Melatonin Administration on Intestinal Injury Caused by Abdominal Irradiation from Mice

doi: 10.3390/ijms22189715

Figure Lengend Snippet: qRT-PCR validation of seven candidate DEGs. ( A ) The expression levels of the target genes of TRIM24 and S100A9 in the microarray data. ( B ) The expression levels of the target genes of TRIM24 and S100A9 validated in the qRT-PCR. Data are represented as mean ± SD, n = 3 mice/group. a p < 0.05 vs. Ctr; b p < 0.05 vs. Rad. NOS2, nitric oxide synthase 2, inducible; VEGFA, vascular endothelial growth factor A; DDC, dopa decarboxylase; VCAN, versican; LGALS3, lectin, galactose binding, soluble 3; IFNG, interferon gamma; TLR2, toll-like receptor 2.

Article Snippet: The aminoallyl-RNA probes were labelled with Cy5 hybridized for 16 h at 50 °C to a mouse oligo microarray (Mouse Whole Genome OneArray MOA2.1; Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Microarray, Binding Assay

Journal: Antioxidants

Article Title: Gallic Acid Improves Diabetic Steatosis by Downregulating MicroRNA-34a-5p through Targeting NFE2L2 Expression in High-Fat Diet-Fed db/db Mice

doi: 10.3390/antiox11010092

Figure Lengend Snippet: miRNA microarray validation with qRT-PCR analysis in liver tissue samples from grouped HFD-fed db/db mice and standard diet-fed db/db mice. The 6-week-old male db/db mice were fed with HFD for 12 weeks. After euthanization, liver tissue samples were obtained for the determination of miRNA expression. miRNAs were selected from the grouped miRNA microarray dataset and examined through qRT-PCR. The miRNA were sorted on the basis of log2 fold changes in miRNA expression and were represented as horizontal bars (red line in figure). ( A ) The volcano plot of liver miRNA expression in HFD (G8) versus db (G2). Standard selection criteria to identify differentially expressed genes were established at log2 |fold change| ≥ 0.585 and a p -value of < 0.05 (blue dots in figure). ( B ) Histogram of log2 |fold change| (HFD versus db). Error bars represent the standard deviation of the mean ± SD. db, db/db mice fed with standard diet; HFD, db/db mice fed with HFD.

Article Snippet: We performed miRNA microarray validation (The Mouse & Rat miRNA OneArray ® , Phalanx Biotech Group, Hsinchu, Taiwan) and qRT-PCR analysis to compare the expression of miRNAs in the HFD-fed db/db mice and standard diet–fed db/db mice [ ].

Techniques: Microarray, Biomarker Discovery, Quantitative RT-PCR, Expressing, Selection, Standard Deviation

Journal: Antioxidants

Article Title: Gallic Acid Improves Diabetic Steatosis by Downregulating MicroRNA-34a-5p through Targeting NFE2L2 Expression in High-Fat Diet-Fed db/db Mice

doi: 10.3390/antiox11010092

Figure Lengend Snippet: Top 10 upregulated and downregulated miRNA expression in the liver tissue from HFD-fed db/db mice. ( a ) A subset of differential genes was selected for clustering analysis. ( b ) Representation of top 10 upregulated and downregulated genes in red and green colors. miRNAs were detected both by microarray analysis between HFD-fed db/db mice and standard diet-fed db/db mice. The corresponding log2 fold changes in miRNA abundance for each miRNA expression level as determined by microarray analysis are shown.

Article Snippet: We performed miRNA microarray validation (The Mouse & Rat miRNA OneArray ® , Phalanx Biotech Group, Hsinchu, Taiwan) and qRT-PCR analysis to compare the expression of miRNAs in the HFD-fed db/db mice and standard diet–fed db/db mice [ ].

Techniques: Expressing, Microarray